Products related to Sequencing:
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Next Generation Sequencing & Applications
Billions of spots of tiny genetic code comprise the human genome.It was DNA sequencing technology that had revolutionized genomic research by decoding the valuable genetic information by giving the picture of an exact order of occurrence of nucleotides in a DNA. The inception of first-generation sequencing method, also called Sanger sequencing took place in 1975.The first major breakthrough of first-generation sequencing comes, when the 13 year log Human Genome Project (HGP) was completed in 2003 at a cost $3 million.With ever increasing demands of researchers and clinicians, complex genomic research require a depth of information which is however beyond the capacity of traditional DNA sequencing technologies.These research questions gaps are very well addressed by Next-generation sequencing (NGS) has filled that gap of cheaper as well as faster sequencing technology. It is just a decade old technology, but it has popularize the next-generation sequencing to high-throughput sequencing hat allow millions to trillions of observations to be made in parallel during a single instrument run.Since the introduction of these technologies, the number of applications and methods that influence the power of genome-scale sequencing has increased exponentially.Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice. The following chapter will highlight the concepts, technologies, and methods of next-generation sequencing to illustrate the breadth and depth of the applications and research areas that are driving progress in genomics.
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Sequencing the Primary Curriculum
This book is an introduction to the primary curriculum for all trainee and early career teachers.It shows how to sequence and plan your teaching in every curriculum subject to ensure you are offering balanced and cohesive learning opportunities that align with the National Curriculum in England. Build your subject knowledge in line with the National CurriculumSequence your teaching so that key curriculum content is threaded through all your lesson plansExplore the basics of curriculum design to support your professional development and help children to learn and remember more over time This is essential reading for trainee teachers on primary initial teacher education courses including university-based (PGCE, BEd, BA with QTS); school-based (School Direct, SCITT, Teaching Apprenticeships) routes into teaching, and early career and experienced teachers wishing to enhance their practice.
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Connecting Camels Sequencing Cards
Connecting Camels Sequencing cards for use with Connecting Camels sets. Connecting Camels are sold separately. Contents 20 laminated colour cards graded from levels 1 to 5 and teachers notes.
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Nanopore Sequencing: An Introduction
This is an introductory text and laboratory manual to be used primarily in undergraduate courses.It is also useful for graduate students and research scientists who require an introduction to the theory and methods of nanopore sequencing.The book has clear explanations of the principles of this emerging technology, together with instructional material written by experts that describes how to use a MinION nanopore instrument for sequencing in research or the classroom.At Harvard University the book serves as a textbook and lab manual for a university laboratory course designed to intensify the intellectual experience of incoming undergraduates while exploring biology as a field of concentration.Nanopore sequencing is an ideal topic as a path to encourage students about the range of courses they will take in Biology by pre-emptively addressing the complaint about having to take a course in Physics or Maths while majoring in Biology.The book addresses this complaint by concretely demonstrating the range of topics - from electricity to biochemistry, protein structure, molecular engineering, and informatics - that a student will have to master in subsequent courses if he or she is to become a scientist who truly understands what his or her biology instrument is measuring when investigating biological phenomena.
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What are the differences between Sanger DNA sequencing and NGS sequencing using a sequencer?
Sanger DNA sequencing is a traditional method that involves sequencing one DNA fragment at a time using chain-terminating dideoxynucleotides. It is a slower and more labor-intensive process compared to NGS sequencing. NGS sequencing, on the other hand, uses massively parallel sequencing technology to simultaneously sequence millions of DNA fragments. This allows for high-throughput sequencing and the generation of large amounts of data in a shorter amount of time. Additionally, NGS sequencing can provide more comprehensive and detailed information about the entire genome, making it more suitable for large-scale genomic studies.
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What is the DNA sequencing for guanine?
The DNA sequencing for guanine is represented by the letter "G". Guanine is one of the four nucleobases found in DNA, along with adenine, cytosine, and thymine. It pairs with cytosine through three hydrogen bonds in the DNA double helix structure. The specific sequence of guanine, along with the other nucleobases, forms the genetic code that determines the characteristics and functions of an organism.
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Why are modern DNA sequencing methods faster?
Modern DNA sequencing methods are faster due to advancements in technology and automation. High-throughput sequencing machines can process multiple samples simultaneously, increasing the speed of data generation. Additionally, improvements in chemistry and bioinformatics have streamlined the sequencing process, reducing the time and resources required for analysis. These advancements have made it possible to sequence large genomes in a fraction of the time it would have taken with older methods, revolutionizing the field of genomics.
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What is the difference between sequencing and transposition?
Sequencing is the process of determining the precise order of nucleotides in a DNA or RNA molecule. It involves identifying the sequence of bases (A, T, C, G) in a specific region of genetic material. Transposition, on the other hand, is a genetic process where a segment of DNA moves from one location in the genome to another. This can result in genetic mutations or changes in the expression of certain genes. In summary, sequencing involves determining the order of nucleotides, while transposition involves the movement of genetic material within the genome.
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Magnetic Colour Sequencing Bugs
The Magnetic Colour Sequencing Bugs are ideal for teaching patterns and sequencing skills.Children select a work card and complete the sequence on their card by joining together the magnetic bug heads and body segments with a simple snap.The cards
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Genotyping by Sequencing for Crop Improvement
OGENOTYPING BY SEQUENCING FOR CROP IMPROVEMENT A thoroughly up-to-date exploration of genotyping-by-sequencing technologies and related methods in plant science In Genotyping by Sequencing for Crop Improvement, a team of distinguished researchers delivers an in-depth and current exploration of the latest advances in genotyping-by-sequencing (GBS) methods, the statistical approaches used to analyze GBS data, and its applications, including quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection (GS) in crop improvement.This edited volume includes insightful contributions on a variety of relevant topics, like advanced molecular markers, high-throughput genotyping platforms, whole genome resequencing, QTL mapping with advanced mapping populations, analytical pipelines for GBS analysis, and more.The distinguished contributors explore traditional and advanced markers used in plant genotyping in extensive detail, and advanced genotyping platforms that cater to unique research purposes are discussed, as is the whole-genome resequencing (WGR) methodology.The included chapters also examine the applications of these technologies in several different crop categories, including cereals, pulses, oilseeds, and commercial crops.Genotyping by Sequencing for Crop Improvement also offers: A thorough introduction to molecular marker techniques and recent advancements in the technologyComprehensive explorations of the genotyping of seeds while preserving their viability, as well as advances in genomic selectionPractical discussions of opportunities and challenges relating to high throughput genotyping in polyploid cropsIn-depth examinations of recent advances and applications of GBS, GWAS, and GS in cereals, pulses, oilseeds, millets, and commercial crops Perfect for practicing plant scientists with an interest in genotyping-by-sequencing technology, Genotyping by Sequencing for Crop Improvement will also earn a place in the libraries of researchers and students seeking a one-stop reference on the foundational aspects of – and recent advances in – genotyping-by-sequencing, genome-wide association studies, and genomic selection.
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Single Cell Sequencing and Systems Immunology
The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology.Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies.It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
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Next Generation Sequencing and Data Analysis
This textbook provides step-by-step protocols and detailed explanations for RNA Sequencing, ChIP-Sequencing and Epigenetic Sequencing applications.The reader learns how to perform Next Generation Sequencing data analysis, how to interpret and visualize the data, and acquires knowledge on the statistical background of the used software tools.Written for biomedical scientists and medical students, this textbook enables the end user to perform and comprehend various Next Generation Sequencing applications and their analytics without prior understanding in bioinformatics or computer sciences.
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What is the difference between these two sequencing methods?
The difference between Sanger sequencing and next-generation sequencing (NGS) lies in their technology and throughput. Sanger sequencing, also known as first-generation sequencing, is a traditional method that uses chain-terminating dideoxynucleotides to sequence DNA. It is a slower and more labor-intensive process, typically used for sequencing shorter DNA fragments. On the other hand, NGS is a high-throughput method that sequences millions of DNA fragments in parallel, allowing for faster and more cost-effective sequencing of entire genomes or targeted regions. NGS also provides greater depth of coverage and can detect rare genetic variants more effectively than Sanger sequencing.
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What is the difference between DNA hybridization and DNA sequencing?
DNA hybridization is a technique used to determine the similarity between two DNA sequences by allowing them to bind together based on complementary base pairing. This method provides information on the degree of similarity between the sequences. On the other hand, DNA sequencing is a technique used to determine the exact order of nucleotides in a DNA molecule. This method provides the precise sequence of the DNA, allowing for detailed analysis of genetic information.
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Why is only a single primer used in DNA sequencing?
Only a single primer is used in DNA sequencing because the primer binds to a specific region of the DNA template, initiating the synthesis of the new DNA strand. This primer is complementary to the template DNA, allowing for the specific amplification of the target region. Using a single primer simplifies the sequencing process and ensures that only the desired region of DNA is amplified and sequenced.
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Is DNA sequencing and DNA sequence analysis the same thing?
No, DNA sequencing and DNA sequence analysis are not the same thing. DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule, while DNA sequence analysis involves interpreting and analyzing the data obtained from DNA sequencing to identify genes, mutations, or other genetic information. In other words, DNA sequencing is the method used to generate the DNA sequence data, while DNA sequence analysis is the process of interpreting and making sense of that data.
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